Review



biotinylated goat anti rabbit igg secondary antibody  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Vector Laboratories biotinylated goat anti rabbit igg secondary antibody
    Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 19583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13059316-74-7-15
    Average 96 stars, based on 19583 article reviews
    biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment
    Article Snippet: For immunohistochemical detection of type I and type II collagen, sections were blocked with 2% bovine serum albumin (BSA) in PBS and subsequently incubated for 1 h at room temperature with either a rabbit monoclonal anti-collagen type I antibody (1:4000, ab138492; Abcam, Cambridge, UK) or a rabbit polyclonal anti-collagen type II antibody (1:100, ab34712; Abcam), both diluted in 5% PBS–BSA. .. After washing with PBS containing Tween 20, sections were incubated for 30 min with a biotinylated anti-rabbit IgG secondary antibody (1:200, VC-BA-1000-MM15; Vector Laboratories, Burlingame, CA, USA) diluted in 2% PBS–BSA, followed by a 30 min incubation with an avidin–biotin–peroxidase complex. .. Peroxidase activity was visualized using diaminobenzidine (Vector Laboratories) as the chromogenic substrate.

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment
    Article Snippet: For immunohistochemical detection of type I and type II collagen, sections were blocked with 2% bovine serum albumin (BSA) in PBS and subsequently incubated for 1 h at room temperature with either a rabbit monoclonal anti-collagen type I antibody (1:4000, ab138492; Abcam, Cambridge, UK) or a rabbit polyclonal anti-collagen type II antibody (1:100, ab34712; Abcam), both diluted in 5% PBS–BSA. .. After washing with PBS containing Tween 20, sections were incubated for 30 min with a biotinylated anti-rabbit IgG secondary antibody (1:200, VC-BA-1000-MM15; Vector Laboratories, Burlingame, CA, USA) diluted in 2% PBS–BSA, followed by a 30 min incubation with an avidin–biotin–peroxidase complex. .. Peroxidase activity was visualized using diaminobenzidine (Vector Laboratories) as the chromogenic substrate.

    Article Title: Endothelial ADAM17 Promotes Neutrophil Migration and Pulmonary Microvascular Permeability in ARDS
    Article Snippet: For immunohistochemical analysis, sections were first blocked with Avidin solution (Vector Laboratories, USA) and then incubated overnight at 4 °C with a rat anti-mouse Ly6G/C antibody (1:500, clone RB6-8C5, Abcam, UK). .. After washing, sections were incubated for 1 hour with a biotinylated anti-rabbit IgG secondary antibody (BA400, Vector Laboratories) and visualization with 3,3’-diaminobenzidine (DAB) substrate (SK-4100, Vector Laboratories). .. Nuclear counterstaining was performed with Mayeŕs hematoxylin (1.09249, Sigma Aldrich, Germany).

    Article Title: HDAC3 Regulates Transcriptional Networks Governing Decidualization
    Article Snippet: Dewaxed, hydrated paraffin- embedded tissue sections were blocked with 10% normal goat serum (NGS, v/v, Vector Laboratories) in phosphate- buffered saline (PBS, pH 7.5) and then incubated overnight at 4°C with rabbit primary antibody anti- LIMK1 (1:500 dilution; 3842S; Cell Signaling), antiPRKAG1 (1:500 dilution; A7300; Abclonal), or anti- CBX2 (1:500 dilution; 18687S; Cell Signaling) diluted in 10% NGS. .. The following day, sections were incubated with biotinylated anti- rabbit IgG secondary antibody (1:500 dilution; BA- 1000; Vector Laboratories) for 1 h at room temperature, followed by incubation with horseradish peroxidase (1:1000 dilution; 43– 4323; Invitrogen) for 45 min. After PBS washes, immunoreactivity was detected using the Vectastain Elite DAB kit (Vector Laboratories). ..

    Avidin-Biotin Assay:

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment
    Article Snippet: For immunohistochemical detection of type I and type II collagen, sections were blocked with 2% bovine serum albumin (BSA) in PBS and subsequently incubated for 1 h at room temperature with either a rabbit monoclonal anti-collagen type I antibody (1:4000, ab138492; Abcam, Cambridge, UK) or a rabbit polyclonal anti-collagen type II antibody (1:100, ab34712; Abcam), both diluted in 5% PBS–BSA. .. After washing with PBS containing Tween 20, sections were incubated for 30 min with a biotinylated anti-rabbit IgG secondary antibody (1:200, VC-BA-1000-MM15; Vector Laboratories, Burlingame, CA, USA) diluted in 2% PBS–BSA, followed by a 30 min incubation with an avidin–biotin–peroxidase complex. .. Peroxidase activity was visualized using diaminobenzidine (Vector Laboratories) as the chromogenic substrate.

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment
    Article Snippet: For immunohistochemical detection of type I and type II collagen, sections were blocked with 2% bovine serum albumin (BSA) in PBS and subsequently incubated for 1 h at room temperature with either a rabbit monoclonal anti-collagen type I antibody (1:4000, ab138492; Abcam, Cambridge, UK) or a rabbit polyclonal anti-collagen type II antibody (1:100, ab34712; Abcam), both diluted in 5% PBS–BSA. .. After washing with PBS containing Tween 20, sections were incubated for 30 min with a biotinylated anti-rabbit IgG secondary antibody (1:200, VC-BA-1000-MM15; Vector Laboratories, Burlingame, CA, USA) diluted in 2% PBS–BSA, followed by a 30 min incubation with an avidin–biotin–peroxidase complex. .. Peroxidase activity was visualized using diaminobenzidine (Vector Laboratories) as the chromogenic substrate.

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 °C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 ◦C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    cDNA Synthesis:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    SYBR Green Assay:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Microscopy:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Imaging:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Capillary Electrochromatography:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Software:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w/v, Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: .. The following principal reagents and materials were used in this study: Isoflo (isoflurane 100% w / v , Zoetis, Parsippany-Troy Hills, NJ, USA); Fluorescein (Fluoralfa, Alfa Intes, Casoria, Naples, Italy); TRI Reagent (Thermo Fisher Scientific, Waltham, MA, USA); iScriptTM cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA); SsoAdvancedTM Universal SYBR ® Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and QuantStudioTM 5 Real-Time PCR System (Thermo Fisher Scientific, Whaltam, MA, USA); hematoxylin–eosin (Harris’s Hematoxylin, Cat. No. 01HEMH2500; Eosin, Cat. No. 01EOY101000; Histo-Line Laboratories, Pantigliate, MI, Italy); Alcian blue (pH 2.5; Cat. No. 04-160802; Bio-Optica, Milan, Italy); periodic acid–Schiff (PAS, Cat. No. 04-130802; Bio-Optica, Milan, Italy); BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany); Tween-20 (Cat. No. P9416; Sigma-Aldrich); biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA); avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK); Complement C3 Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA); Complement C5a Polyclonal Antibody (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), optical microscope (Eclipse E600; Nikon, Shinjuku, Japan); Imaging Source “33” Series USB 3.0 camera (Cat. No. DFK 33UX264; Bremen, Germany); Grundium Ocus 20 scanner (Tampere, Finland); ImageJ software (version 1.53a; National Institutes of Health, Bethesda, MD, USA); Controlled-Environment Chamber (CEC) (Laboratory Products Inc., Seaford, DE); GraphPad Prism software (version 10.0 or 8.0.2; GraphPad Software, San Diego, CA, USA); QuPath 0.6.0 software (Free Open Source software). ..

    Binding Assay:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 °C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 ◦C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Blocking Assay:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 °C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 ◦C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Amplification:

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 °C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..

    Article Title: Integrated Evaluation of Corneal Damage, Goblet Cell Remodeling and Inflammatory Response in a Murine Model of Environmental Dry Eye Disease (DED)
    Article Snippet: Sections were then allowed to cool to room temperature for 20 min. For each protocol, tissue sections were incubated overnight at 4 ◦C in a humidified chamber with primary antibodies against C3 (rabbit polyclonal antibody, Cat. No. PAS-21349; Invitrogen, Rockford, IL, USA) or C5a (rabbit polyclonal antibody, Cat. No. PAS-78891; Invitrogen, Rockford, IL, USA), both diluted 1:200 in a solution containing 3% BSA (Cat. No. A9418; Sigma-Aldrich, Darmstadt, Germany) and 0.25% Tween-20 (Cat. No. P9416; Sigma-Aldrich) in PBS (0.01 M, pH 7.2). .. Binding sites were detected using a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Newark, CA, USA; 1:200 dilution in blocking solution, 30 min at room temperature) and amplified with a commercial avidin–biotin–peroxidase complex (VECTASTAIN ABC Kit, Peterborough, UK). ..



    Similar Products

    biotinylated goat anti rabbit igg secondary antibody  (Vector Laboratories)
    96
    Vector Laboratories biotinylated goat anti rabbit igg secondary antibody
    Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13059316-74-7-15
    Average 96 stars, based on 1 article reviews
    biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotinylated secondary antibody  (Vector Laboratories)
    96
    Vector Laboratories biotinylated secondary antibody
    Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Horse+Anti-Rabbit+IgG+Antibody/pmc13050099-77-7-11
    Average 96 stars, based on 1 article reviews
    biotinylated secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti rabbit mouse igg secondary antibodies  (Vector Laboratories)
    96
    Vector Laboratories anti rabbit mouse igg secondary antibodies
    Anti Rabbit Mouse Igg Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13062622-169-6-14
    Average 96 stars, based on 1 article reviews
    anti rabbit mouse igg secondary antibodies - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    goat anti rabbit biotinylated secondary antibody  (Jackson Immuno)
    93
    Jackson Immuno goat anti rabbit biotinylated secondary antibody
    Goat Anti Rabbit Biotinylated Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Rabbit+IgG%2C+Fc+fragment+specific/pmc12996694-111-14-22
    Average 93 stars, based on 1 article reviews
    goat anti rabbit biotinylated secondary antibody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    anti rabbit igg secondary antibody  (Vector Laboratories)
    96
    Vector Laboratories anti rabbit igg secondary antibody
    Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pm42059278-103-26-34
    Average 96 stars, based on 1 article reviews
    anti rabbit igg secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    biotinylated goat anti rabbit secondary antibody  (Vector Laboratories)
    96
    Vector Laboratories biotinylated goat anti rabbit secondary antibody
    Biotinylated Goat Anti Rabbit Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13132197-111-17-23
    Average 96 stars, based on 1 article reviews
    biotinylated goat anti rabbit secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    secondary antibodies  (Vector Laboratories)
    96
    Vector Laboratories secondary antibodies
    Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/bio_rxiv__64898__2026__04__23__720463-59-18-22
    Average 96 stars, based on 1 article reviews
    secondary antibodies - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    secondary antibody  (Vector Laboratories)
    96
    Vector Laboratories secondary antibody
    Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+rabbit+igg+secondary+antibody/Biotinylated+Rabbit+Anti-Goat+IgG+Antibody/bio_rxiv__64898__2026__04__23__720315-81-23-28
    Average 96 stars, based on 1 article reviews
    secondary antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results